Welcome!

Welcome to Trio-One, a certified DOT Clinic operating within the offices of Advanced Chiropractic.

We offer the convenience of a one stop facility to accommodate the needs of Drug Testing and DOT physicals.

  • DOT physicals, Drug Screening for companies and individuals.
  • Non-DOT Pre-employment and Drug Screenings.
  • We will manage and administer your random drug testing and help keep your company DOT Compliant. Our Third Party Administrator will handle your compliance needs..
  • Lowest Available Rates in Knoxville guaranteed for driver or employer. We want your business!
  • Drug Testing Services available in our clinic or on site at your business.  Upon request, in most cases, we can test at your business.
  • Easy access and location with plenty of parking.  We have large spaces for big rigs.
  • We have same day appointments, with under 30 minutes for Drug Testing.  Call us!
  • Laminated medical cards upon completion of DOT Physicals.

At Your Service


TRIO-ONE Certified DOT Facility

7349 Chapman Highway

Knoxville, TN 37920

Tel:  (865) 579-6500

Fax: (865) 579-7985


(Across from Wal-Mart on Chapman Highway)


Office Hours

Monday
7:30 a.m. - 5:00 p.m.

Tuesday
7:30 a.m. - 3:00 p.m.

Wednesday
7:30 a.m. - 6:00 p.m.

Thursday
7:30 a.m. - 5:00 p.m.

Friday
Available on request